Reproducible consistent amplification across a wide dynamic range fast sybr green master mix contains all of the components excluding the template and primers in a convenient 2x master mix amplitaq fast dna polymerase up a highly purified dna polymerase designed to allow instant hot start minimizing non specific product formation. The kapa sybr fast qpcr kit is supplied as a 2x master mix with integrated antibody mediated hot start sybr green i fluorescent dye mgcl 2 dntps and stabilizers. Kits include kapa sybr fast qpcr master mix 2x which contains kapa sybr fast dna polymerase reaction buffer dntps sybr green i dye and mgcl 2 at a final concentration of 2 5 mm where noted master mixes contain instrument specific reference dyes while the universal kit includes rox high and rox low both 50x separately.
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